Using Surfactants to Prevent Protein Aggregation in Pharmaceuticals
In-class teacher demonstration
For the teacher:
- 1 standard size paper plate
- 25 mL water
- less than 2 g of ground black pepper
- 5-10 drops of liquid dish soap (Dawn or similar)
For the class to share
- laptop or computer with projector (to display Exploring Surfactants Presentation to class)
Part 1: Investigating surface tension and surfactants
Note: To test the surface pressures of different chemicals, each group will need to assemble a testing apparatus. This activity provides two options: Method 1 is a low-cost DIY version that will collect more general qualitative data, while Method 2 uses a scientific triple-beam balance (which may be available in some classrooms) to collect more precise quantitative data.
Method 1: Building a low-cost DIY surface tension balance
Each group needs:
- 3 18 oz. plastic cups
- 1 small weigh boat
- 1 spool of thread
- 1 sewing needle
- 2 small wooden dowels or chopsticks
- 1 pair of scissors
- 1 utility knife
- 1 permanent marker
- 1 ruler with units in centimeters
- modeling clay
- tape
- water, preferably distilled
- 2 g SLSA powder
- weigh boat
- spoon or stirring rod
- 100 mL graduated cylinder
- 1 ml bulb pipette
- permanent marker
- paper towels
- (optional) 1 electronic balance
For the entire class to share:
- 1 electronic balance
Method 2: Assembling a scientific beam balance apparatus
Each group needs:
- 1 50 mL beaker
- 1 spool of thread
- 1 medium plastic button (preferably flat-faced, 4 holes, approximately 17-20 mm in diameter)
- 1 ruler with units in centimeters
- (optional) 1 triple-beam pan balance (e.g., Ohaus Cent-O-Gramâ„¢ Balance or similar)
For the class to share
- 1 triple-beam pan balance (e.g., Ohaus Cent-O-Gramâ„¢ Balance or similar)
Part 2: Investigating protein aggregation solutions
Each group needs:
- 50 mL of four mystery solutions (labeled A, B, C, D) for testing. Each solution should be prepared at a 4% concentration using one of the following compounds: a surfactant (such as SLSA powder), a stabilizer (such as sugar), or potentially disruptive agents (such as borax or salt).
- 1 hand mixer with 2 eggbeater attachments
- 100 mL egg whites
- 5 small containers with transparent lids
- 1 bowl large enough to mix solutions in
- 5-10 pipettes
- 50 mL water, preferably distilled
https://www.teachengineering.org/activities/view/uok-2910-surfactants-protein-aggregation-pharmaceuticals-activity