Materials List: Stop the Stick:
Using Surfactants to Prevent Protein Aggregation in Pharmaceuticals

In-class teacher demonstration

For the teacher:

  • 1 standard size paper plate
  • 25 mL water
  • less than 2 g of ground black pepper
  • 5-10 drops of liquid dish soap (Dawn or similar)

For the class to share

Part 1: Investigating surface tension and surfactants

Note: To test the surface pressures of different chemicals, each group will need to assemble a testing apparatus. This activity provides two options: Method 1 is a low-cost DIY version that will collect more general qualitative data, while Method 2 uses a scientific triple-beam balance (which may be available in some classrooms) to collect more precise quantitative data.

Method 1: Building a low-cost DIY surface tension balance

Each group needs:

  • 3 18 oz. plastic cups
  • 1 small weigh boat
  • 1 spool of thread
  • 1 sewing needle
  • 2 small wooden dowels or chopsticks
  • 1 pair of scissors
  • 1 utility knife
  • 1 permanent marker
  • 1 ruler with units in centimeters
  • modeling clay
  • tape
  • water, preferably distilled
  • 2 g SLSA powder
  • weigh boat
  • spoon or stirring rod
  • 100 mL graduated cylinder
  • 1 ml bulb pipette
  • permanent marker
  • paper towels
  • (optional) 1 electronic balance

For the entire class to share:

  • 1 electronic balance

Method 2: Assembling a scientific beam balance apparatus

Each group needs:

  • 1 50 mL beaker
  • 1 spool of thread
  • 1 medium plastic button (preferably flat-faced, 4 holes, approximately 17-20 mm in diameter)
  • 1 ruler with units in centimeters
  • (optional) 1 triple-beam pan balance (e.g., Ohaus Cent-O-Gramâ„¢ Balance or similar)

For the class to share

  • 1 triple-beam pan balance (e.g., Ohaus Cent-O-Gramâ„¢ Balance or similar)

Part 2: Investigating protein aggregation solutions

Each group needs:

  • 50 mL of four mystery solutions (labeled A, B, C, D) for testing. Each solution should be prepared at a 4% concentration using one of the following compounds: a surfactant (such as SLSA powder), a stabilizer (such as sugar), or potentially disruptive agents (such as borax or salt).
  • 1 hand mixer with 2 eggbeater attachments
  • 100 mL egg whites
  • 5 small containers with transparent lids
  • 1 bowl large enough to mix solutions in
  • 5-10 pipettes
  • 50 mL water, preferably distilled
https://www.teachengineering.org/activities/view/uok-2910-surfactants-protein-aggregation-pharmaceuticals-activity