Day 1

Each group needs:

• 1000 µL micropipette, available online individually or in a set of three
• 1000 µLmicropipette tip, available in a box of 100 or a box of 1000
• 15 ml culture tubes, available online
• 1 µL tip inoculating loops, available online (two per group)
• test tube rack
• Student Lab Sheet Part 1 (one per student)

To share with the entire class:

• petri dish of E. coli (to be shared among three groups), available online
• 30 ml of LB Broth bacteria growth medium, available online
• 1000 ml jar with re-sealable lid
• 250 ml beaker (or similar container to hold anaerobic culture tubes upright in the re-sealable jar)
• test tube rack secured onto a tabletop shaker (to hold the aerobic tubes)
• tabletop shaker (capable of 200 rpm), available online
• electric tape
• tea candle
• one bottle of bleach disinfectant (to spray materials when finished)
• multipurpose lighter
• lab coats, goggles, and disposable gloves for each student

Day 2

Each group needs:

• 1000 µL micropipette
• two 1000 µL micropipettes micropipette tips
• ultrafine point marker
• two 1.5 ml micro-centrifuge tubes, available online
• micro-tube or test tube rack
• bottle of bleach disinfectant (to spray materials when finished)
• lab coats, goggles, and disposable gloves for each student
• Student Lab Sheet Part 2 (one per student)
• poster paper (one sheet for every 2 students)
• Student Rubric Part 3 (one per every 2 students)

To share with the entire class:

• micro-centrifuge (capable of 6000 rcf and hold at least 16 micro-tubes)
• bottle of bleach disinfectant (to spray materials when finished)
• markers and/or color pencils
• sticky notes
• internet-capable devices